Design and validation of a homogeneous time-resolved fluorescence-based leptin receptor binding assay.

Vauthier, Virginie; Derviaux, Carine; Douayry, Najim; Roux, Thomas; Trinquet, Eric; Jockers, Ralf; Dam, Julie

Vauthier, Virginie; Derviaux, Carine; Douayry, Najim; Roux, Thomas; Trinquet, Eric; Jockers, Ralf; Dam, Julie.

Affiliation

Vauthier V; Institut National de la Santé et de la Recherche Médicale (INSERM), U1016, Institut Cochin, 75014 Paris, France.

Anal Biochem ; 436(1): 1-9, 2013 May 01.

Article in En | MEDLINE | ID: mdl-23333588

Subject(s)

Fluorescence; Receptors, Leptin/metabolism; Alkaline Phosphatase/chemistry; Alkaline Phosphatase/metabolism; Binding Sites/drug effects; Cells, Cultured; HEK293 Cells; High-Throughput Screening Assays; Hormone Antagonists/chemistry; Hormone Antagonists/metabolism; Hormone Antagonists/pharmacology; Humans; Leptin/antagonists & inhibitors; Leptin/chemistry; Leptin/metabolism; Ligands; Protein Binding; Receptors, Leptin/analysis; Reproducibility of Results; Time Factors

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Receptors, Leptin / Fluorescence Type of study: Prognostic_studies Limits: Humans Language: En Journal: Anal Biochem Year: 2013 Document type: Article Affiliation country: France Country of publication: United States

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