Visualization of inactive X chromosome in preimplantation embryos utilizing MacroH2A-EGFP transgenic mouse.
Genesis
; 51(4): 259-67, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-23349035
ABSTRACT
One of the two X chromosomes is inactivated in female eutherian mammals. MacroH2A, an unusual histone variant, is known to accumulate on the inactive X chromosome (Xi) during early embryo development, and can thus be used as a marker of the Xi. In this study, we produced a transgenic mouse line expressing the mouse MacroH2A1.2-enhanced green fluorescent protein (EGFP) fusion protein (MacroH2A-EGFP) under the control of a CAG promoter and verified whether MacroH2A-EGFP would be useful for tracing the process of X chromosome inactivation by visualizing Xi noninvasively in preimplantation embryos. In transgenic female mice, MacroH2A-EGFP formed a fluorescent focus in nuclei throughout the body. In female blastocysts, the MacroH2A-EGFP focus colocalized with Xist RNA, well known as a marker of Xi. Fluorescence marking of Xi was first observed in some embryonic cells between the 4- and 8-cell stages. These results demonstrate that MacroH2A can bind to the Xi by around the 8-cell stage in female mouse embryos. These MacroH2A-EGFP transgenic mice might be useful to elucidate the process of X chromosome inactivation during the mouse life cycle.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Blastocyst
/
Histones
/
Green Fluorescent Proteins
/
X Chromosome Inactivation
Limits:
Animals
Language:
En
Journal:
Genesis
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Document type:
Article
Affiliation country:
Japan