[Scepter--the use of automated cell counter in quantitative and qualitative analysis of human peripheral blood mononuclear cells]. / Scepter--zastosowanie automatycznego licznika komórek w ilosciowej i jakosciowej ocenie komórek mononuklearnych krwi obwodowej czlowieka.

ABSTRACT

Scepter is easy to use and convenient tool for rapid cell counting in in vitro culture. Quantitative and qualitative results are presented immediately after the measurement on its screen. So far, Bürker's chamber was most commonly used for the counting of cells. Due to the presence of a small display, Scepter as the flow cytometers reveals data on cell diameter and volume in the form of histograms. It facilitates the differentiation of various cell populations in the studied samples. This article presents experiments designed to isolate and compare peripheral blood mononuclear cells (PBMCs) from children and adults. To this end, the both of mentioned tools were used.