Clinical application of Sleeping Beauty and artificial antigen presenting cells to genetically modify T cells from peripheral and umbilical cord blood.

Huls, M Helen; Figliola, Matthew J; Dawson, Margaret J; Olivares, Simon; Kebriaei, Partow; Shpall, Elizabeth J; Champlin, Richard E; Singh, Harjeet; Cooper, Laurence J N

Huls, M Helen; Figliola, Matthew J; Dawson, Margaret J; Olivares, Simon; Kebriaei, Partow; Shpall, Elizabeth J; Champlin, Richard E; Singh, Harjeet; Cooper, Laurence J N.

Affiliation

Huls MH; Division of Pediatrics, U.T. MD Anderson Cancer Center, TX, USA.

J Vis Exp ; (72): e50070, 2013 Feb 01.

Article in En | MEDLINE | ID: mdl-23407473

Subject(s)

Antigen-Presenting Cells/cytology; Fetal Blood/cytology; T-Lymphocytes/physiology; Transposases/genetics; Antigen Presentation; DNA, Superhelical/genetics; Electroporation; Humans; K562 Cells; Plasmids/genetics; Receptors, Antigen, T-Cell; T-Lymphocytes/immunology; Transposases/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: T-Lymphocytes / Transposases / Fetal Blood / Antigen-Presenting Cells Limits: Humans Language: En Journal: J Vis Exp Year: 2013 Document type: Article Affiliation country: United States Country of publication: United States

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