Increased bronchial density of CD25+Foxp3+ regulatory T cells in occupational asthma: relationship to current smoking.
Scand J Immunol
; 77(5): 398-404, 2013 May.
Article
in En
| MEDLINE
| ID: mdl-23421612
ABSTRACT
To identify activated T cell subset in the asthmatic bronchia, we developed a triple-colour immunohistofluorescence labelling technique on cryo-section to discriminate activated CD4+CD25+ T cells, (effector T cells) from Foxp3+ regulatory T cells (Treg). Additional coexpression of activation and proliferation markers was also examined in situ. Bronchial biopsies were taken from 20 aluminium potroom workers (12 smokers) with asthma (>12% reversibility), 15 non-asthmatic potroom workers (7 smokers) and 10 non-smoking, non-exposed controls. Non-smoking asthmatics had significantly higher subepithelial density of both Tregs, effector T cells, activated (HLA-DR+) CD8+ and activated CD4+ T cells. Moreover, both Tregs, effector T cells and CD8+ T cells proliferated in the non-smoking asthmatics, only. Although smoking asthmatics had no asthma-associated increase in bronchial T cell, both had a significantly increase in effector T cell to Treg ratios. The significantly increased bronchial density of Tregs, effector T cells, proliferative T cells and activated CD8+ T cells in non-smoking asthmatics clearly showed that both the effector T cells and the inhibitory Treg system were activated in asthma.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Bronchi
/
Smoking
/
T-Lymphocytes, Regulatory
/
Asthma, Occupational
Type of study:
Prognostic_studies
Limits:
Adult
/
Humans
/
Middle aged
Language:
En
Journal:
Scand J Immunol
Year:
2013
Document type:
Article
Affiliation country:
Norway