Sequence-specific electron injection into DNA from an intermolecular electron donor.
Nucleic Acids Res
; 41(8): 4724-8, 2013 Apr.
Article
in En
| MEDLINE
| ID: mdl-23439569
ABSTRACT
Electron transfer in DNA has been intensively studied to elucidate its biological roles and for applications in bottom-up DNA nanotechnology. Recently, mechanisms of electron transfer to DNA have been investigated; however, most of the systems designed are intramolecular. Here, we synthesized pyrene-conjugated pyrrole-imidazole polyamides (PPIs) to achieve sequence-specific electron injection into DNA in an intermolecular fashion. Electron injection from PPIs into DNA was detected using 5-bromouracil as an electron acceptor. Twelve different 5-bromouracil-containing oligomers were synthesized to examine the electron-injection ability of PPI. Product analysis demonstrated that the electron transfer from PPIs was localized in a range of 8 bp from the binding site of the PPIs. These results demonstrate that PPIs can be a useful tool for sequence-specific electron injection.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
DNA
/
Electrons
/
Nylons
Language:
En
Journal:
Nucleic Acids Res
Year:
2013
Document type:
Article
Affiliation country:
Japan