Study on the fluorescence quenching reaction of amitriptyline and clomipramine hydrochlorides with eosin Y and its analytical application.
J Fluoresc
; 23(3): 533-42, 2013 May.
Article
in En
| MEDLINE
| ID: mdl-23456417
ABSTRACT
Amitriptyline.HCl (AMI) and clomipramine.HCl (CMI) react with eosin Y (EY) in pH 3.8 NaAc-AcH buffer solution to form ion association complex which results in quenching of fluorescence of EY and appearance of a new resonance Rayleigh scattering (RSS) spectrum at 620 nm. The spectral characteristics of absorption, fluorescence and RSS spectra have been investigated. The factors influencing the reaction were studied and optimum conditions for the reaction have been determined. Based on fluorescence quenching, a simple and sensitive spectrofluorimetric method for determination of AMI and CMI has been developed. The fluorescence quenching intensity was measured at 550 nm using an excitation wavelength of 310 nm. The calibration graph was found to be rectilinear in the range 0.08-2.0 µg mL(-1) with detection limit of 0.017 µg mL(-1) for AMI and 0.06-2.0 µg mL(-1) with detection limit of 0.015 µg mL(-1) for CMI. The method can be satisfactorily applied to the determination of AMI and CMI in tablets without interference from commonly occurring exicipients. The recovery and RSD values obtained indicate good accuracy and precision of the method. The mechanism of the reaction and fluorescence quenching has also been discussed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Clomipramine
/
Eosine Yellowish-(YS)
/
Fluorescent Dyes
/
Amitriptyline
Language:
En
Journal:
J Fluoresc
Journal subject:
BIOFISICA
Year:
2013
Document type:
Article
Affiliation country:
India