Epitope mapping of antibodies by mass spectroscopy: a case study.

Obungu, Victor H; Gelfanova, Valentina; Huang, Lihua

Obungu, Victor H; Gelfanova, Valentina; Huang, Lihua.

Affiliation

Obungu VH; Lilly Research Laboratories, Indianapolis, IN, USA.

Methods Mol Biol ; 988: 291-302, 2013.

Article in En | MEDLINE | ID: mdl-23475727

Subject(s)

Antibodies, Monoclonal/chemistry; Epitopes/chemistry; Animals; Biotinylation/methods; CHO Cells; Chromatography, High Pressure Liquid; Cricetinae; Deuterium Exchange Measurement; Epitope Mapping/methods; Epitopes/isolation & purification; Fas Ligand Protein/chemistry; Fas Ligand Protein/isolation & purification; Humans; Immobilized Proteins/chemistry; Immobilized Proteins/isolation & purification; Peptide Fragments/chemistry; Peptide Fragments/isolation & purification; Proteolysis; Recombinant Proteins/chemistry; Recombinant Proteins/isolation & purification; Solutions; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods; Trypsin/chemistry

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Antibodies, Monoclonal / Epitopes Type of study: Prognostic_studies Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2013 Document type: Article Affiliation country: United States Country of publication: United States

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