Malignant transformation by a eukaryotic initiation factor subunit that binds to mRNA 5' cap.
Nature
; 345(6275): 544-7, 1990 Jun 07.
Article
in En
| MEDLINE
| ID: mdl-2348862
ABSTRACT
Eukaryotic cellular mRNAs have a 5' cap structure (m7 GpppX) that facilitates binding to ribosomes and is required for efficient translation. A specific initiation factor, eIF-4F, mediates the function of the cap and consists of three subunits, one of which, eIF-4E, binds the cap. This subunit is present in limiting amounts in the cell, and is thought to be regulated by phosphorylation decreased phosphorylation of eIF-4E following various treatments correlates with a decrease in cellular translation rate. These observations suggest that eIF-4E lies on the mitogenic signal transduction pathway, and we reasoned that overexpression of eIF-4E might profoundly affect cellular growth properties. We report here that overexpression of eIF-4E in NIH 3T3 and Rat 2 fibroblasts causes their tumorigenic transformation as determined by three criteria formation of transformed foci on a monolayer of cells; anchorage-independent growth; and tumour formation in nude mice.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Caps
/
Cell Transformation, Neoplastic
/
Peptide Initiation Factors
Limits:
Animals
Language:
En
Journal:
Nature
Year:
1990
Document type:
Article
Affiliation country:
Canada