Extracellular ß-fructofuranosidase from Fusarium graminearum: stability of the spray-dried enzyme in the presence of different carbohydrates.
J Microencapsul
; 30(7): 624-31, 2013.
Article
in En
| MEDLINE
| ID: mdl-23489014
ABSTRACT
Microbial enzymes have been used for various biotechnological applications; however, enzyme stabilization remains a challenge for industries and needs to be considered. This study describes the effects of spray-drying conditions on the activity and stability of ß-fructofuranosidase from Fusarium graminearum. The extracellular enzyme ß-fructofuranosidase was spray dried in the presence of stabilizers, including starch (Capsul) (SC), microcrystalline cellulose (MC), trehalose (TR), lactose (LC) and ß-cyclodextrin (CD). In the presence of TR (2% w/v), the enzymatic activity was fully retained. After 1 year of storage, 74% of the enzymatic activity was maintained with the CD stabilizer (10% w/v). The residual activity was maintained as high as 80% for 1 h at 70°C when MC, SC and CD (5% w/v) stabilizers were used. Spray drying with carbohydrates was effective in stabilizing the F. graminearum ß-fructofuranosidase, improved enzymatic properties compared to the soluble enzyme and demonstrated a potential use in future biotechnology applications.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Beta-Fructofuranosidase
/
Fusarium
Language:
En
Journal:
J Microencapsul
Journal subject:
FARMACIA
Year:
2013
Document type:
Article
Affiliation country:
Brazil