[Detection of the expression of NK ligands in acute leukemia cell lines by real-time PCR].

ABSTRACT

OBJECTIVE: To detect the expression profile of NK ligands in acute leukemia cell lines and investigate the differential expression pattern between acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). METHODS: Using quantitative real-time PCR, 23 NK ligands (MICA, MICB, ULBP-1, ULBP-2, ULBP-3, ULBP-4, HLA-E, HLA-G, CD48, NBTA, HLA-F, LLT-1, PVR, Nectin2, CD72, CD80, ICAM-1, LFA-3, CRACC, Fas, DR4, DR5, TNFR1) were detected in 6 acute leukemia cell lines, including 3 ALL cell lines (CEM, Jurkat T, Reh) and 3 AML cell lines (HL-60, KG-1a, NB4), respectively. Independent-samples t test analysis was performed to determine statistical significance. RESULTS: Using ß-actin as reference gene, the relative expression results showed that the expression of 4 NK ligands between ALL and AML is significantly different. Specifically, the level of ULBP-2 is higher in ALL (CEM 1, Jurkat T 0.617, Reh 0.246) than that in AML (HL-60 0.000, KG-1a 0.003, NB4 0.000)(P = 0.047). However, the expressions of CD48, PVR(PVR-1, PVR-2) and DR4 is higher in AML (HL-60 13.987, 4.403, 10.334, 8.711; KG-1a 5.387, 2.900, 7.315, 4.512; NB4 7.763, 3.248, 7.049, 6.127) than that in ALL (CEM 1, 1, 1, 1; Jurkat T 2.035, 1.553, 3.888, 0.449; Reh 1.559, 0.000, 0.000, 1.304) (P = 0.044, 0.014, 0.014, 0.011). And there're no significant differences between the rest 19 NK ligands. CONCLUSIONS: ULBP-2, CD48, PVR and DR4 might play an important role in the distinct mechanisms in leukemogenesis between ALL and AML and could be potential targets for diagnosis and treatment.