Igs expressed by chronic lymphocytic leukemia B cells show limited binding-site structure variability.
J Immunol
; 190(11): 5771-8, 2013 Jun 01.
Article
in En
| MEDLINE
| ID: mdl-23636053
ABSTRACT
Ag selection has been suggested to play a role in chronic lymphocytic leukemia (CLL) pathogenesis, but no large-scale analysis has been performed so far on the structure of the Ag-binding sites (ABSs) of leukemic cell Igs. We sequenced both H and L chain V(D)J rearrangements from 366 CLL patients and modeled their three-dimensional structures. The resulting ABS structures were clustered into a small number of discrete sets, each containing ABSs with similar shapes and physicochemical properties. This structural classification correlates well with other known prognostic factors such as Ig mutation status and recurrent (stereotyped) receptors, but it shows a better prognostic value, at least in the case of one structural cluster for which clinical data were available. These findings suggest, for the first time, to our knowledge, on the basis of a structural analysis of the Ab-binding sites, that selection by a finite quota of antigenic structures operates on most CLL cases, whether mutated or unmutated.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Immunoglobulins
/
Leukemia, Lymphocytic, Chronic, B-Cell
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
J Immunol
Year:
2013
Document type:
Article
Affiliation country:
Italy
Publication country:
EEUU
/
ESTADOS UNIDOS
/
ESTADOS UNIDOS DA AMERICA
/
EUA
/
UNITED STATES
/
UNITED STATES OF AMERICA
/
US
/
USA