Stealth cationic liposomes modified with anti-CAGE single-chain fragment variable deliver recombinant methioninase for gastric carcinoma therapy.
J Nanosci Nanotechnol
; 13(1): 178-83, 2013 Jan.
Article
in En
| MEDLINE
| ID: mdl-23646714
ABSTRACT
Stealth cationic liposomes (SCLs) modified with tumor-targeting single-chain fragment variable (scFV) antibody for systemic delivery of recombinant methioninase (rMETase) for gastric cancer were prepared successfully. These functional SCL nanoparticles are composed of cationic lipids, dioleoylphosphatidylethanolamine, and distearoylphosphatidylethanolamine-polyethylene glycol, which have lower gene transfection efficiencies compared with Lipofectamine 2000, and can also be used as effective gene delivery vectors. Increased therapeutic efficiency of rMETase-loaded scFV-SCLs were tested in SGC-7901 cells and compared with free rMETase in solution and rMETase-loaded SCLs. In addition, scFV-SCLs (effective diameter 185.7 nm; polydispersity index 0.236) can significantly boost rhodamine 123 cellular accumulation in the cytoplasm. The scFV-targeted SCLs can be used as a potentially effective drug delivery system.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stomach Neoplasms
/
Carbon-Sulfur Lyases
/
Serine Endopeptidases
/
Nanocapsules
/
Single-Chain Antibodies
/
Liposomes
Limits:
Humans
Language:
En
Journal:
J Nanosci Nanotechnol
Year:
2013
Document type:
Article
Affiliation country:
China