Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound.
Cell Prolif
; 46(3): 320-7, 2013 Jun.
Article
in En
| MEDLINE
| ID: mdl-23692090
ABSTRACT
OBJECTIVES:
Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters. MATERIALS ANDMETHODS:
Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence.RESULTS:
Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated.CONCLUSIONS:
We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Osteogenesis
/
Sound
/
Cell Differentiation
/
Adipose Tissue
/
Adipocytes
/
Mesenchymal Stem Cells
Limits:
Animals
Language:
En
Journal:
Cell Prolif
Year:
2013
Document type:
Article
Affiliation country:
China