A continuous spectrophotometric assay for adenosine 5'-phosphosulfate reductase activity with sulfite-selective probes.

Paritala, Hanumantharao; Carroll, Kate S

Paritala, Hanumantharao; Carroll, Kate S.

Affiliation

Paritala H; Department of Chemistry, The Scripps Research Institute, Jupiter, FL 33458, USA.

Anal Biochem ; 440(1): 32-9, 2013 Sep 01.

Article in En | MEDLINE | ID: mdl-23711725

Subject(s)

Fluorescent Dyes/chemistry; Levulinic Acids/analysis; Mycobacterium tuberculosis/metabolism; Oxidoreductases Acting on Sulfur Group Donors/analysis; Spectrophotometry/methods; Sulfites/analysis; Fluorescent Dyes/analysis; Kinetics; Mutagenesis; Sensitivity and Specificity

Key words

Adenosine 5'-phosphosulfate (APS); Adenosine 5'-phosphosulfate reductase (APR); Levulinate; Sulfate assimilation; Sulfite sensor

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Spectrophotometry / Sulfites / Oxidoreductases Acting on Sulfur Group Donors / Fluorescent Dyes / Levulinic Acids / Mycobacterium tuberculosis Type of study: Diagnostic_studies Language: En Journal: Anal Biochem Year: 2013 Document type: Article Affiliation country: United States

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