Effects of recombinant human AMH on SCF expression in human granulosa cells.
Cell Biochem Biophys
; 67(3): 1481-5, 2013.
Article
in En
| MEDLINE
| ID: mdl-23723001
ABSTRACT
The aim of the present study was to investigate the effects of recombinant human anti-mullerian hormone (rhAMH) on Stem Cell Factor (SCF) expression in human granulosa cells (GCs). GCs were obtained from infertile patients undergoing IVF-ET cycles and cultured with 20 ng/ml of rhAMH. The levels of SCF mRNA and protein were detected in both matched and experimental group by real-time PCR, immunofluorescence, and ELISA, respectively, on day 4 of culture. We found that human GCs expressed SCF mRNA and protein, and SCF expression in the experimental group was significantly lower than that in the matched group (p < 0.05). We further showed that rhAMH inhibited SCF expression at mRNA and protein levels.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Stem Cell Factor
/
Anti-Mullerian Hormone
/
Granulosa Cells
Limits:
Adult
/
Female
/
Humans
Language:
En
Journal:
Cell Biochem Biophys
Journal subject:
BIOFISICA
/
BIOQUIMICA
Year:
2013
Document type:
Article