Flash-induced consumption of molecular oxygen on the donor side of photosystem II in Mn-depleted subchloroplast membrane fragments: specific effects of manganese and calcium ions.
Photosynth Res
; 117(1-3): 367-74, 2013 Nov.
Article
in En
| MEDLINE
| ID: mdl-23756831
ABSTRACT
It has been shown that removal of manganese from the water-oxidizing complex (WOC) of photosystem II (PSII) leads to flash-induced oxygen consumption (FIOC) which is activated by low concentration of Mn(2+) (Yanykin et al., Biochim Biophys Acta 1797516-523, 2010). In the present work, we examined the effect of transition and non-transition divalent metal ions on FIOC in Mn-depleted PSII (apo-WOC-PSII) preparations. It was shown that only Mn(2+) ions are able to activate FIOC while other transition metal ions (Fe(2+), V(2+) and Cr(2+)) capable of electron donation to the apo-WOC-PSII suppressed the photoconsumption of O2. Co(2+) ions with a high redox potential (E (0) for Co(2+)/Co(3+) is 1.8 V) showed no effect. Non-transition metal ions Ca(2+) by Mg(2+) did not stimulate FIOC. However, Ca(2+) (in contrast to Mg(2+)) showed an additional activation effect in the presence of exogenic Mn(2+). The Ca(2+) effect depended on the concentration of both Mn(2+) and Ca(2+). The Ca effect was only observed when (1) the activation of FIOC induced by Mn(2+) did not reach its maximum, (2) the concentration of Ca(2+) did not exceed 40 µM; at higher concentrations Ca(2+) inhibited the Mn(2+)-activated O2 photoconsumption. Replacement of Ca(2+) by Mg(2+) led to a suppression of Mn(2+)-activated O2 photoconsumption; while, addition of Ca(2+) resulted in elimination of the Mg(2+) inhibitory effect and activation of FIOC. Thus, only Mn(2+) and Ca(2+) (which are constituents of the WOC) have specific effects of activation of FIOC in apo-WOC-PSII preparations. Possible reactions involving Mn(2+) and Ca(2+) which could lead to the activation of FIOC in the apo-WOC-PSII are discussed.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxygen Consumption
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Chloroplasts
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Calcium
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Spinacia oleracea
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Photosystem II Protein Complex
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Intracellular Membranes
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Manganese
Language:
En
Journal:
Photosynth Res
Journal subject:
METABOLISMO
Year:
2013
Document type:
Article
Affiliation country:
RUSSIA