Confirmation and 3D profiling of anabolic steroid esters in injection sites using imaging desorption electrospray ionisation (DESI) mass spectrometry.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
; 30(6): 1012-9, 2013.
Article
in En
| MEDLINE
| ID: mdl-23767976
ABSTRACT
In this study, desorption electrospray ionisation (DESI) linear ion trap tandem mass spectrometry (MS(n)) was applied for the confirmation and three-dimensional profiling of anabolic steroid esters in an injection site of bovine muscle. The spatial resolution of the DESI-MS(n) was demonstrated by scanning hormone esters and marker ink lines drawn at various distances on a microscopic slide at set distances, using an x-scanner with manual y and z adjustment. Tissue slices of bovine muscle injected with a hormone cocktail were analysed. All anabolic steroid esters could be directly detected in the sample and confirmed on the basis of identification points awarded for selected MS/MS transitions according to the performance criteria given in Commission Decision 2002/657/EC. Moreover, the injection site could be mapped by two-dimensional and three-dimensional imaging MS, showing a horizontal and vertical distribution through the muscle tissue. This DESI approach offers potential for analysis of injection sites of steroid esters from illegally treated animals; moreover, direct analysis by ambient imaging DESI-MS still allows conventional extraction and analysis of the whole tissue for further confirmatory or contra-analysis afterwards.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Steroids
/
Spectrometry, Mass, Electrospray Ionization
/
Anabolic Agents
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
Journal subject:
CIENCIAS DA NUTRICAO
Year:
2013
Document type:
Article
Affiliation country:
Netherlands
Publication country:
ENGLAND
/
ESCOCIA
/
GB
/
GREAT BRITAIN
/
INGLATERRA
/
REINO UNIDO
/
SCOTLAND
/
UK
/
UNITED KINGDOM