Chimerism testing by quantitative PCR using Indel markers.
Methods Mol Biol
; 1034: 221-37, 2013.
Article
in En
| MEDLINE
| ID: mdl-23775739
ABSTRACT
Engraftment monitoring is critical for patients after Hematopoietic Stem Cell Transplantation (HSCT). Complete donor chimerism is the goal; therefore, early detection of rejection and relapse is crucial for guiding the patient post HSCT treatment. Quantitative PCR for chimerism testing has been reported to be highly sensitive. In this chapter we discuss the quantitative PCR (qPCR) method using 34 Indel (Insertion and Deletion) genetic markers spread over 20 different chromosomes.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Markers
/
Transplantation Chimera
/
Graft Rejection
/
Molecular Biology
Type of study:
Screening_studies
Limits:
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2013
Document type:
Article
Affiliation country:
United States