Site-specific NMR mapping and time-resolved monitoring of serine and threonine phosphorylation in reconstituted kinase reactions and mammalian cell extracts.

Theillet, Francois-Xavier; Rose, Honor May; Liokatis, Stamatios; Binolfi, Andres; Thongwichian, Rossukon; Stuiver, Marchel; Selenko, Philipp

Theillet, Francois-Xavier; Rose, Honor May; Liokatis, Stamatios; Binolfi, Andres; Thongwichian, Rossukon; Stuiver, Marchel; Selenko, Philipp.

Affiliation

Theillet FX; In-Cell NMR Laboratory, Department of NMR-supported Structural Biology, Leibniz Institute of Molecular Pharmacology (FMP Berlin), Berlin, Germany.

Nat Protoc ; 8(7): 1416-32, 2013.

Article in En | MEDLINE | ID: mdl-23807285

Subject(s)

Cell Extracts/analysis; Nuclear Magnetic Resonance, Biomolecular/methods; Proteins/metabolism; Serine/metabolism; Threonine/metabolism; Animals; Humans; Phosphorylation; Protein Kinases/chemistry; Protein Kinases/metabolism; Protein Structure, Tertiary; Proteins/analysis; Serine/analysis; Threonine/analysis; Time Factors; Tumor Suppressor Protein p53/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Serine / Threonine / Proteins / Cell Extracts / Nuclear Magnetic Resonance, Biomolecular Limits: Animals / Humans Language: En Journal: Nat Protoc Year: 2013 Document type: Article Affiliation country: Germany Country of publication: United kingdom

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