Electrophoretic mobility-shift assays.
Cold Spring Harb Protoc
; 2013(7): 636-9, 2013 Jul 01.
Article
in En
| MEDLINE
| ID: mdl-23818676
ABSTRACT
In an electrophoretic mobility-shift assay (EMSA, or simply "gel shift"), a (32)P-labeled DNA fragment containing a specific DNA site is incubated with a cognate DNA-binding protein. The protein-DNA complexes are separated from free (unbound) DNA by electrophoresis through a nondenaturing polyacrylamide gel. The protein retards the mobility of the DNA fragments to which it binds. Thus, the free DNA will migrate faster than the DNA-protein complex. An image of the gel is used to reveal the positions of the free and bound radiolabeled DNAs.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Electrophoretic Mobility Shift Assay
Language:
En
Journal:
Cold Spring Harb Protoc
Year:
2013
Document type:
Article