Molecular engineering of organophosphate hydrolysis activity from a weak promiscuous lactonase template.

Meier, Monika M; Rajendran, Chitra; Malisi, Christoph; Fox, Nicholas G; Xu, Chengfu; Schlee, Sandra; Barondeau, David P; Höcker, Birte; Sterner, Reinhard; Raushel, Frank M

Meier, Monika M; Rajendran, Chitra; Malisi, Christoph; Fox, Nicholas G; Xu, Chengfu; Schlee, Sandra; Barondeau, David P; Höcker, Birte; Sterner, Reinhard; Raushel, Frank M.

Affiliation

Meier MM; Institute of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany.

J Am Chem Soc ; 135(31): 11670-7, 2013 Aug 07.

Article in En | MEDLINE | ID: mdl-23837603

Subject(s)

Organophosphates/metabolism; Phosphoric Triester Hydrolases/genetics; Phosphoric Triester Hydrolases/metabolism; Pseudomonas/enzymology; Pseudomonas/metabolism; Carboxylic Ester Hydrolases/chemistry; Carboxylic Ester Hydrolases/genetics; Carboxylic Ester Hydrolases/metabolism; Crystallography, X-Ray; Hydrolysis; Models, Molecular; Mutagenesis; Phosphoric Triester Hydrolases/chemistry; Protein Conformation; Pseudomonas/chemistry; Pseudomonas/genetics; Stereoisomerism; Substrate Specificity

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Organophosphates / Pseudomonas / Phosphoric Triester Hydrolases Language: En Journal: J Am Chem Soc Year: 2013 Document type: Article Affiliation country: Germany Country of publication: United States

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