A sensitive fluorigenic substrate for selective in vitro and in vivo assay of leukotriene A4 hydrolase activity.

Poras, Hervé; Duquesnoy, Sophie; Fournié-Zaluski, Marie-Claude; Ratinaud-Giraud, Céline; Roques, Bernard P; Ouimet, Tanja

Poras, Hervé; Duquesnoy, Sophie; Fournié-Zaluski, Marie-Claude; Ratinaud-Giraud, Céline; Roques, Bernard P; Ouimet, Tanja.

Affiliation

Poras H; Pharmaleads, Paris BioPark, 75013 Paris, France.

Anal Biochem ; 441(2): 152-61, 2013 Oct 15.

Article in En | MEDLINE | ID: mdl-23851339

Subject(s)

Enzyme Assays/methods; Epoxide Hydrolases/antagonists & inhibitors; Epoxide Hydrolases/metabolism; Naphthalenes/metabolism; Peptides/metabolism; Animals; Enzyme Inhibitors/pharmacology; Epoxide Hydrolases/chemistry; Humans; Male; Mice; Molecular Docking Simulation; Naphthalenes/analysis; Peptides/analysis; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/metabolism; Spectrometry, Fluorescence/methods; Substrate Specificity

Key words

Aminopeptidase; In vivo enzyme assay; LTA4 hydrolase; Specific inhibitor; Specific substrate

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Epoxide Hydrolases / Enzyme Assays / Naphthalenes Type of study: Diagnostic_studies Limits: Animals / Humans / Male Language: En Journal: Anal Biochem Year: 2013 Document type: Article Affiliation country: France

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