Value of HIV patients with regular follow-up as in-house internal controls of flow cytometry measurement of lymphocyte subsets.
Cytometry B Clin Cytom
; 84(5): 324-6, 2013.
Article
in En
| MEDLINE
| ID: mdl-23929698
ABSTRACT
BACKGROUND:
Quality assessment in flow cytometry cannot obey the same rules as those applicable to the measurement of chemical analytes. However, regular follow-up of known patients may provide a robust in-house control of cell subsets evaluation.METHODS:
Sequential blood samples assessed for 32 HIV patients over several years and showing good stability were retrospectively assessed to establish coefficient of variations of the percentages of CD3+, CD4+, CD8+ cells, and CD4+ absolute counts (ACs).RESULTS:
Mean relative standard variations for the whole cohort were of 0.04, 0.14, 0.08, and 0.18 for CD3%, CD4%, CD8%, and CD4 ACs, respectively.DISCUSSION:
In-house follow-up of regularly checked compliant patients is a good alternative to traditional and costly repeatability and reproducibility studies for the validation of routine flow cytometry. © 2013 International Clinical Cytometry Society.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
CD4-Positive T-Lymphocytes
/
HIV Infections
/
CD8-Positive T-Lymphocytes
/
Flow Cytometry
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
Limits:
Adult
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
Cytometry B Clin Cytom
Year:
2013
Document type:
Article
Affiliation country:
France