Manufacturing porcine islets: culture at 22 °C has no advantage above culture at 37 °C: a gene expression evaluation.

Mueller, Kate R; Martins, Kyra V; Murtaugh, Michael P; Schuurman, Henk-Jan; Papas, Klearchos K

Manufacturing porcine islets: culture at 22 °C has no advantage above culture at 37 °C: a gene expression evaluation.

Mueller, Kate R; Martins, Kyra V; Murtaugh, Michael P; Schuurman, Henk-Jan; Papas, Klearchos K.

Affiliation

Mueller KR; Department of Surgery, Schulze Diabetes Institute, University of Minnesota, Minneapolis, MN, USA.

Xenotransplantation ; 20(6): 418-28, 2013.

Article in En | MEDLINE | ID: mdl-23941232

Subject(s)

Islets of Langerhans/immunology; Islets of Langerhans/metabolism; Sus scrofa/immunology; Tissue Culture Techniques/methods; Animals; Chemokines/genetics; Female; Gene Expression; Genetic Markers; Heterografts; Humans; Islets of Langerhans Transplantation/immunology; Islets of Langerhans Transplantation/methods; Lymphocytes/immunology; Lymphocytes/metabolism; Lymphokines/genetics; Oxygen Consumption; Temperature; Tissue and Organ Harvesting/methods

Key words

apoptotic markers; chemokines; cytokines; islet culture; lymphocyte markers; porcine islets; temperature

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Islets of Langerhans / Sus scrofa / Tissue Culture Techniques Type of study: Evaluation_studies Limits: Animals / Female / Humans Language: En Journal: Xenotransplantation Journal subject: TRANSPLANTE Year: 2013 Document type: Article Affiliation country: United States Country of publication: Denmark

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