Towards the rational design of antimicrobial proteins: single point mutations can switch on bactericidal and agglutinating activities on the RNase A superfamily lineage.

Pulido, David; Moussaoui, Mohammed; Nogués, M Victòria; Torrent, Marc; Boix, Ester

Pulido, David; Moussaoui, Mohammed; Nogués, M Victòria; Torrent, Marc; Boix, Ester.

Affiliation

Pulido D; Department of Biochemistry and Molecular Biology, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain.

FEBS J ; 280(22): 5841-52, 2013 Nov.

Article in En | MEDLINE | ID: mdl-23992292

Subject(s)

Antimicrobial Cationic Peptides/genetics; Antimicrobial Cationic Peptides/immunology; Protein Engineering/methods; Ribonuclease, Pancreatic/genetics; Ribonuclease, Pancreatic/immunology; Agglutinins/genetics; Agglutinins/immunology; Animals; Antimicrobial Cationic Peptides/chemistry; Endoribonucleases/chemistry; Endoribonucleases/genetics; Endoribonucleases/immunology; Eosinophil Cationic Protein/chemistry; Eosinophil Cationic Protein/genetics; Eosinophil Cationic Protein/immunology; Gram-Negative Bacteria/drug effects; Humans; Models, Molecular; Mutagenesis, Site-Directed; Point Mutation; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/immunology; Ribonuclease, Pancreatic/chemistry

Key words

antimicrobial peptides; eosinophil cationic protein; eosinophil-derived neurotoxin; protein evolution; ribonuclease

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Ribonuclease, Pancreatic / Protein Engineering / Antimicrobial Cationic Peptides Limits: Animals / Humans Language: En Journal: FEBS J Journal subject: BIOQUIMICA Year: 2013 Document type: Article Affiliation country: Spain Country of publication: United kingdom

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