Your browser doesn't support javascript.
loading
Direct conversion of sugars and organic acids to biobutanol by non-growing cells of Clostridium spp. incubated in a nitrogen-free medium.
Loyarkat, Sawang; Cheirsilp, Benjamas; Umsakul, Kamontam.
Affiliation
  • Loyarkat S; Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand.
Appl Biochem Biotechnol ; 171(7): 1726-38, 2013 Dec.
Article in En | MEDLINE | ID: mdl-23996124
ABSTRACT
Several Clostridium spp. were incubated in a nitrogen-free medium (non-growth medium) containing only butyric acid as a sole precursor for performing butanol production by non-growing cells. Non-growing cells of Clostridium spp., especially Clostridium beijerinckii TISTR 1461, could convert butyric acid to butanol via their sole solventogenic activity. This activity was further enhanced in the presence of glucose as a co-substrate. In addition to glucose, other monosaccharides (i.e., galactose and xylose) and disaccharides (i.e., maltose, sucrose, and lactose) could also be used as a co-substrate with butyric acid. Among the organic acids tested (i.e., formic, acetic, propionic, and butyric acids), only butyric and acetic acids were converted to butanol. This study has shown that it is possible to use the non-growing cells of Clostridium spp. for direct conversion of sugars and organic acids to biobutanol. With this strategy, C. beijerinckii TISTR 1461 produced 12 g/L butanol from 15 g/L glucose and 10 g/L butyric acid with a high butanol yield of 0.68 C-mol/C-mol and a high butanol ratio of 88 %.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acids, Acyclic / Clostridium / Culture Media / Disaccharides / Butanols / Monosaccharides Language: En Journal: Appl Biochem Biotechnol Year: 2013 Document type: Article Affiliation country: Thailand

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Acids, Acyclic / Clostridium / Culture Media / Disaccharides / Butanols / Monosaccharides Language: En Journal: Appl Biochem Biotechnol Year: 2013 Document type: Article Affiliation country: Thailand