Validation of dilution of plasma samples with phosphate buffered saline to eliminate the problem of small volumes associated with children infected with HIV-1 for viral load testing using Cobas AmpliPrep/COBAS TaqMan HIV-1 test, version 2.0 (CAP CTM HIV v2.0).

Mine, Madisa; Nkoane, Tapologo; Sebetso, Gaseene; Sakyi, Bright; Makhaola, Kgomotso; Gaolathe, Tendani

Mine, Madisa; Nkoane, Tapologo; Sebetso, Gaseene; Sakyi, Bright; Makhaola, Kgomotso; Gaolathe, Tendani.

Affiliation

Mine M; Botswana Harvard HIV Reference Laboratory, Princess Marina Hospital, 1836 North Ring Road, Gaborone, Botswana; National Health Laboratory, 5353 Church Road, Extension 10, Gaborone, Botswana. Electronic address: mmine@bhp.org.bw.

J Virol Methods ; 194(1-2): 217-21, 2013 Dec.

Article in En | MEDLINE | ID: mdl-24025342

Subject(s)

HIV Infections/diagnosis; HIV Infections/virology; HIV-1/isolation & purification; Molecular Diagnostic Techniques/methods; Plasma/virology; Specimen Handling/methods; Viral Load/methods; Botswana; Buffers; Child; Humans; Phosphates; Sodium Chloride

Key words

COBAS TaqMan HIV-1 test; Dilution; HIV-1 RNA; Viral load

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Plasma / Specimen Handling / HIV Infections / HIV-1 / Viral Load / Molecular Diagnostic Techniques Type of study: Diagnostic_studies / Evaluation_studies / Guideline / Risk_factors_studies Limits: Child / Humans Country/Region as subject: Africa Language: En Journal: J Virol Methods Year: 2013 Document type: Article Country of publication: Netherlands

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google