The structural organization of the N-terminus domain of SopB, a virulence factor of Salmonella, depends on the nature of its protein partners.
Biochim Biophys Acta
; 1834(12): 2564-72, 2013 Dec.
Article
in En
| MEDLINE
| ID: mdl-24075929
ABSTRACT
The TTSS is used by Salmonella and many bacterial pathogens to inject virulence factors directly into the cytoplasm of target eukaryotic cells. Once translocated these so-called effector proteins hijack a vast array of crucial cellular functions to the benefit of the bacteria. In the bacterial cytoplasm, some effectors are stabilized and maintained in a secretion competent state by interaction with specific type III chaperones. In this work we studied the conformation of the Chaperone Binding Domain of the effector named Salmonella Outer protein B (SopB) alone and in complex with its cognate chaperone SigE by a combination of biochemical, biophysical and structural approaches. Our results show that the N-terminus part of SopB is mainly composed by α-helices and unfolded regions whose organization/stabilization depends on their interaction with the different partners. This suggests that the partially unfolded state of this N-terminal region, which confers the adaptability of the effector to bind very different partners during the infection cycle, allows the bacteria to modulate numerous host cells functions limiting the number of translocated effectors.
Key words
Bacterial virulence; CBD; CD; Chaperone binding domain; DSP; Intrinsically disordered protein; R(g); R(h); SAXS; Salmonella outer protein B; Small angle X-ray scattering; SopB; TTSS; Type three secretion system; chaperone binding domain; circular dichroism; dithio-bis-(succinimidylpropionate); hydrodynamic radius; radius of gyration; small angle X-ray scattering; type three secretion system
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Salmonella typhimurium
/
Sigma Factor
/
Bacterial Proteins
/
Molecular Chaperones
Language:
En
Journal:
Biochim Biophys Acta
Year:
2013
Document type:
Article
Publication country:
HOLANDA
/
HOLLAND
/
NETHERLANDS
/
NL
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PAISES BAJOS
/
THE NETHERLANDS