Reliable PCR quantitation of estrogen, progesterone and ERBB2 receptor mRNA from formalin-fixed, paraffin-embedded tissue is independent of prior macro-dissection.

Tramm, Trine; Hennig, Guido; Kyndi, Marianne; Alsner, Jan; Sørensen, Flemming Brandt; Myhre, Simen; Sørlie, Therese; Overgaard, Jens

Tramm, Trine; Hennig, Guido; Kyndi, Marianne; Alsner, Jan; Sørensen, Flemming Brandt; Myhre, Simen; Sørlie, Therese; Overgaard, Jens.

Affiliation

Tramm T; Department of Experimental Clinical Oncology, Aarhus University Hospital, Nørrebrogade 44, Building 5, 2nd floor, 8000, Aarhus, Denmark, tramm@oncology.dk.

Virchows Arch ; 463(6): 775-86, 2013 Dec.

Article in En | MEDLINE | ID: mdl-24100522

Subject(s)

Biomarkers, Tumor/analysis; Breast Neoplasms/genetics; Gene Expression Profiling/methods; RNA, Messenger/analysis; Real-Time Polymerase Chain Reaction/methods; Biomarkers, Tumor/genetics; Cytodiagnosis/methods; Female; Formaldehyde; Humans; Immunohistochemistry; Paraffin Embedding; Receptor, ErbB-2/analysis; Receptor, ErbB-2/biosynthesis; Receptor, ErbB-2/genetics; Receptors, Estrogen/analysis; Receptors, Estrogen/biosynthesis; Receptors, Estrogen/genetics; Receptors, Progesterone/analysis; Receptors, Progesterone/biosynthesis; Receptors, Progesterone/genetics; Tissue Fixation

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Breast Neoplasms / RNA, Messenger / Biomarkers, Tumor / Gene Expression Profiling / Real-Time Polymerase Chain Reaction Limits: Female / Humans Language: En Journal: Virchows Arch Journal subject: BIOLOGIA MOLECULAR / PATOLOGIA Year: 2013 Document type: Article Country of publication: Germany

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