Purification and characterization of a gelatinolytic matrix metalloproteinase from the skeletal muscle of grass carp (Ctenopharyngodon idellus).

Wu, Jiu-Lin; Ge, Shang-Ying; Cai, Zhi-Xing; Liu, Hui; Liu, Ying-Xiang; Wang, Jian-Hua; Zhang, Qi-Qing

Wu, Jiu-Lin; Ge, Shang-Ying; Cai, Zhi-Xing; Liu, Hui; Liu, Ying-Xiang; Wang, Jian-Hua; Zhang, Qi-Qing.

Affiliation

Wu JL; Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China. Electronic address: wujiul590@126.com.

Food Chem ; 145: 632-8, 2014 Feb 15.

Article in En | MEDLINE | ID: mdl-24128525

Subject(s)

Carps; Gelatin/metabolism; Matrix Metalloproteinases/isolation & purification; Matrix Metalloproteinases/metabolism; Muscle, Skeletal/enzymology; Animals; Calcium/chemistry; Chromatography, Gel; Collagen Type I/metabolism; Edetic Acid/chemistry; Edetic Acid/metabolism; Electrophoresis, Polyacrylamide Gel; Hydrogen-Ion Concentration; Matrix Metalloproteinases/chemistry; Molecular Weight; Peptides/analysis; Protease Inhibitors/chemistry; Protease Inhibitors/metabolism; Protein Binding; Substrate Specificity; Tandem Mass Spectrometry; Temperature

Key words

Gelatin zymography; Grass carp; Matrix metalloproteinase; Post-mortem tenderization; Purification

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Carps / Muscle, Skeletal / Matrix Metalloproteinases / Gelatin Limits: Animals Language: En Journal: Food Chem Year: 2014 Document type: Article

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