Synthesis of biomimetic oxygen-carrying compartmentalized microparticles using flow lithography.
Lab Chip
; 13(24): 4765-74, 2013 Dec 21.
Article
in En
| MEDLINE
| ID: mdl-24141406
ABSTRACT
We report a microfluidic approach for lithographically photo-patterning compartmentalized microparticles with any 2D-extruded shape, down to the cellular length scale (~10 microns). The prepolymer solution consists of a UV crosslinkable perfluorodecalin-in-water nanoemulsion stabilized by Pluronic(®) F-68. The nanoemulsions are generated using high-pressure homogenization and are osmotically stabilized by the trapped species method. The presence of PFC droplets increases the solubility and diffusivity of oxygen in the prepolymer solution, thereby enhancing the rate of O2 inhibition during microparticle synthesis. We develop a simple model that successfully predicts the augmented O2 mass transport, which agrees well with experimental data. Informed by our analytical results, cell-sized composite microgels are generated by controlling the oxygen environment around the polydimethylsiloxane (PDMS) microfluidic synthesis device. These nanoemulsion composites are functionally similar to red blood cells as oxygen carriers. Such bio-inspired polymeric particles with controlled physical properties are promising vehicles for drug delivery and clinical diagnostics.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Oxygen
/
Drug Carriers
/
Biomimetic Materials
/
Microfluidic Analytical Techniques
/
Microspheres
Type of study:
Prognostic_studies
Language:
En
Journal:
Lab Chip
Journal subject:
BIOTECNOLOGIA
/
QUIMICA
Year:
2013
Document type:
Article
Affiliation country:
United States