Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification.
Methods Mol Biol
; 1095: 109-19, 2014.
Article
in En
| MEDLINE
| ID: mdl-24166307
ABSTRACT
The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA Precursors
/
Nucleic Acid Amplification Techniques
/
MicroRNAs
/
DNA Replication
Type of study:
Diagnostic_studies
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2014
Document type:
Article
Affiliation country:
Germany