ITP of lanthanides in microfluidic PMMA chip.
Electrophoresis
; 35(5): 646-53, 2014 Mar.
Article
in En
| MEDLINE
| ID: mdl-24258617
ABSTRACT
An ITP separation of eight lanthanides on a serpentine PMMA microchip with a tee junction and a 230-mm-long serpentine channel is described. The cover of the PMMA chip is 175 µm thick so that a C(4) D in microchip mode can be used to detect the lanthanides as they migrate through the microchannel. Acetate and α-hydroxyisobutyric acid are used as complexing agents to increase the electrophoretic mobility difference between the lanthanides. Eight lanthanides are concentrated within â¼ 6 min by ITP in the microchip using 10 mM ammonium acetate at pH 4.5 as the leading electrolyte and 10 mM acetic acid at â¼ pH 3.0 as the terminating electrolyte. In addition, a 2D numerical simulation of the lanthanides undergoing ITP in the microchip is compared with experimental results using COMSOL Multiphysics v4.3a.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Lanthanoid Series Elements
/
Electrophoresis, Microchip
Language:
En
Journal:
Electrophoresis
Year:
2014
Document type:
Article
Affiliation country:
United States