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Back to translation: removal of aIF2 from the 5'-end of mRNAs by translation recovery factor in the crenarchaeon Sulfolobus solfataricus.
Märtens, Birgit; Manoharadas, Salim; Hasenöhrl, David; Zeichen, Lukas; Bläsi, Udo.
Affiliation
  • Märtens B; Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, Center of Molecular Biology, University of Vienna, Dr. Bohrgasse 9, 1030 Vienna, Austria.
Nucleic Acids Res ; 42(4): 2505-11, 2014 Feb.
Article in En | MEDLINE | ID: mdl-24271401
The translation initiation factor aIF2 of the crenarchaeon Sulfolobus solfataricus (Sso) recruits initiator tRNA to the ribosome and stabilizes mRNAs by binding via the γ-subunit to their 5'-triphosphate end. It has been hypothesized that the latter occurs predominantly during unfavorable growth conditions, and that aIF2 or aIF2-γ is released on relief of nutrient stress to enable in particular anew translation of leaderless mRNAs. As leaderless mRNAs are prevalent in Sso and aIF2-γ bound to the 5'-end of a leaderless RNA inhibited ribosome binding in vitro, we aimed at elucidating the mechanism underlying aIF2/aIF2-γ recycling from mRNAs. We have identified a protein termed Trf (translation recovery factor) that co-purified with trimeric aIF2 during outgrowth of cells from prolonged stationary phase. Subsequent in vitro studies revealed that Trf triggers the release of trimeric aIF2 from RNA, and that Trf directly interacts with the aIF2-γ subunit. The importance of Trf is further underscored by an impaired protein synthesis during outgrowth from stationary phase in a Sso trf deletion mutant.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Chain Initiation, Translational / RNA, Messenger / Archaeal Proteins / Prokaryotic Initiation Factors / Sulfolobus solfataricus Type of study: Prognostic_studies Language: En Journal: Nucleic Acids Res Year: 2014 Document type: Article Affiliation country: Austria Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptide Chain Initiation, Translational / RNA, Messenger / Archaeal Proteins / Prokaryotic Initiation Factors / Sulfolobus solfataricus Type of study: Prognostic_studies Language: En Journal: Nucleic Acids Res Year: 2014 Document type: Article Affiliation country: Austria Country of publication: United kingdom