Predicting the expression of recombinant monoclonal antibodies in Chinese hamster ovary cells based on sequence features of the CDR3 domain.

Pybus, Leon P; James, David C; Dean, Greg; Slidel, Tim; Hardman, Colin; Smith, Andrew; Daramola, Olalekan; Field, Ray

Pybus, Leon P; James, David C; Dean, Greg; Slidel, Tim; Hardman, Colin; Smith, Andrew; Daramola, Olalekan; Field, Ray.

Affiliation

Pybus LP; ChELSI Institute, Dept. of Chemical and Biological Engineering, University of Sheffield, Mappin Street, Sheffield S1 3JD, U.K.

Biotechnol Prog ; 30(1): 188-97, 2014.

Article in En | MEDLINE | ID: mdl-24311306

Subject(s)

Antibodies, Monoclonal/chemistry; Biotechnology/methods; Complementarity Determining Regions/genetics; Computational Biology/methods; Recombinant Proteins/chemistry; Amino Acid Sequence; Animals; Antibodies, Monoclonal/genetics; Antibodies, Monoclonal/metabolism; CHO Cells; Cricetinae; Cricetulus; Hydrophobic and Hydrophilic Interactions; Immunoglobulin Light Chains/chemistry; Immunoglobulin Light Chains/genetics; Immunoglobulin Light Chains/metabolism; RNA, Messenger/genetics; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Sequence Analysis, Protein

Key words

Chinese hamster; bioinformatics; difficult-to-express proteins; ovary cells; recombinant monoclonal antibodies

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Biotechnology / Recombinant Proteins / Computational Biology / Complementarity Determining Regions / Antibodies, Monoclonal Type of study: Prognostic_studies / Risk_factors_studies Limits: Animals Language: En Journal: Biotechnol Prog Journal subject: BIOTECNOLOGIA Year: 2014 Document type: Article Affiliation country: United kingdom Country of publication: United States

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