Correlation of contrast-enhanced ultrasound with two distinct types of blood vessels for the assessment of angiogenesis in lewis lung carcinoma.
Ultraschall Med
; 35(5): 468-72, 2014 Oct.
Article
in En
| MEDLINE
| ID: mdl-24327471
ABSTRACT
OBJECTIVE:
The aim of our study was to evaluate tumor angiogenesis in Lewis lung carcinoma (LLC) of mice using a contrast-enhanced ultrasound (CEUS) examination, and to determine the correlation of contrast-enhanced ultrasonographic parameters with different blood vessel markers of microvessel density (MVD). MATERIALS ANDMETHODS:
Subcutaneous Lewis lung carcinomas were established in 25 mice, which were evaluated by contrast-enhanced US using SonoVue (a second-generation US contrast agent). The results were recorded as digital video images and the time-intensity curves and hemodynamic parameters were analyzed. Pathological tumor specimens were obtained just after US examination. Tumor specimens were stained with hematoxylin and eosin (H & E) and expression of CD31 and CD34, the different endothelial cell markers, was determined by immunohistochemical straining. Then the relationship between the CEUS parameters and the level of MVD was analyzed.RESULTS:
Two distinct types of microvessels were identified in Lewis lung carcinoma differentiated (CD34â+) and undifferentiated (CD31â+) vessels. A significant correlation was found between CEUS parameters and undifferentiated MVD (CD31â+ vessels) in LLC (P <â0.05). There was a reverse correlation between the different MVDs.CONCLUSION:
The study showed that among the distinct types of vasculature (CD31â+ and CD34â+) in Lewis lung carcinoma, the former correlated with the CEUS parameters. Therefore, CEUS using a second-generation US contrast agent may be useful for the evaluation of tumor angiogenesis of LLC of mice.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phospholipids
/
Sulfur Hexafluoride
/
Image Enhancement
/
Carcinoma, Lewis Lung
/
Contrast Media
/
Neovascularization, Pathologic
Type of study:
Diagnostic_studies
Limits:
Animals
Language:
En
Journal:
Ultraschall Med
Year:
2014
Document type:
Article