CUDC-101, a histone deacetylase inhibitor, improves the in vitro and in vivo developmental competence of somatic cell nuclear transfer pig embryos.
Theriogenology
; 81(4): 572-8, 2014 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-24342668
ABSTRACT
The aim of the present study was to examine the effects of CUDC-101, a novel histone deacetylase inhibitor, on the in vitro development and expression of the epigenetic marker histone H3 at lysine 9 (AcH3K9) in pig SCNT embryos. We found that treatment with 1 µmol/L CUDC-101 for 24 hours significantly improved the development of pig SCNT embryos. Compared with the control group, the blastocyst rate was higher (18.5% vs. 10.3%; P < 0.05). To assess in vivo developmental potency, CUDC-101-treated SCNT embryos were transferred into two surrogate mothers, resulting in one pregnancy with six fetuses. We then investigated the acetylation level of histone H3K9 in SCNT embryos treated with CUDC-101 and compared them only against untreated embryos. The acetylation level of control SCNT embryos was lower than that of CUDC-101-treated embryos at pseudo-pronuclear stages, and immunofluorescent signal for H3K9ac in CUDC-101-treated embryos in a pattern similar to that of control group. In conclusion, we demonstrated that CUDC-101 can significantly improve in vitro and in vivo developmental competence and enhance the nuclear reprogramming of pig SCNT embryos.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Quinazolines
/
Swine
/
Histones
/
Embryonic Development
/
Embryo, Mammalian
/
Enzyme Inhibitors
/
Hydroxamic Acids
Limits:
Animals
/
Pregnancy
Language:
En
Journal:
Theriogenology
Year:
2014
Document type:
Article
Affiliation country:
China