Inhibitory effects of ß-cyclodextrin-helenalin complexes on H-TERT gene expression in the T47D breast cancer cell line - results of real time quantitative PCR.
Asian Pac J Cancer Prev
; 14(11): 6949-53, 2013.
Article
in En
| MEDLINE
| ID: mdl-24377631
ABSTRACT
BACKGROUND:
Nowadays, the encapsulation of cytotoxic chemotherapeutic agents is attracting interest as a method for drug delivery. We hypothesized that the efficiency of helenalin might be maximized by encapsulation in ß-cyclodextrin nanoparticles. Helenalin, with a hydrophobic structure obtained from flowers of Arnica chamissonis and Arnica Montana, has anti-cancer and anti-inflammatory activity but low water solubility and bioavailability. ß-Cyclodextrin (ß-CD) is a cyclic oligosaccharide comprising seven D-glucopyranoside units, linked through 1,4-glycosidic bonds. MATERIALS ANDMETHODS:
To test our hypothesis, we prepared ß-cyclodextrin- helenalin complexes to determine their inhibitory effects on telomerase gene expression by real-time polymerase chain reaction (q-PCR) and cytotoxic effects by colorimetric cell viability (MTT) assay.RESULTS:
MTT assay showed that not only ß-cyclodextrin has no cytotoxic effect on its own but also it demonstrated that ß-cyclodextrin- helenalin complexes inhibited the growth of the T47D breast cancer cell line in a time and dose-dependent manner. Our q-PCR results showed that the expression of telomerase gene was effectively reduced as the concentration of ß-cyclodextrin-helenalin complexes increased.CONCLUSIONS:
ß-Cyclodextrin-helenalin complexes exerted cytotoxic effects on T47D cells through down-regulation of telomerase expression and by enhancing Helenalin uptake by cells. Therefore, ß-cyclodextrin could be superior carrier for this kind of hydrophobic agent.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Sesquiterpenes
/
Breast Neoplasms
/
Apoptosis
/
Telomerase
/
Beta-Cyclodextrins
/
Cell Proliferation
/
Antineoplastic Agents, Phytogenic
Limits:
Female
/
Humans
Language:
En
Journal:
Asian Pac J Cancer Prev
Journal subject:
NEOPLASIAS
Year:
2013
Document type:
Article