Optimization of high grade glioma cell culture from surgical specimens for use in clinically relevant animal models and 3D immunochemistry.

Hasselbach, Laura A; Irtenkauf, Susan M; Lemke, Nancy W; Nelson, Kevin K; Berezovsky, Artem D; Carlton, Enoch T; Transou, Andrea D; Mikkelsen, Tom; deCarvalho, Ana C

Hasselbach, Laura A; Irtenkauf, Susan M; Lemke, Nancy W; Nelson, Kevin K; Berezovsky, Artem D; Carlton, Enoch T; Transou, Andrea D; Mikkelsen, Tom; deCarvalho, Ana C.

Affiliation

Hasselbach LA; Department of Neurosurgery, Henry Ford Hospital.

J Vis Exp ; (83): e51088, 2014 Jan 07.

Article in En | MEDLINE | ID: mdl-24429465

Subject(s)

Brain Neoplasms/pathology; Cell Culture Techniques/methods; Glioblastoma/pathology; Neoplastic Stem Cells/pathology; Neural Stem Cells/pathology; Animals; Brain Neoplasms/metabolism; Culture Media, Serum-Free; Glioblastoma/metabolism; Humans; Immunohistochemistry; Mice; Neoplasm Grading; Neoplastic Stem Cells/metabolism; Neural Stem Cells/metabolism; Paraffin Embedding/methods; Spheroids, Cellular

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Neoplastic Stem Cells / Brain Neoplasms / Glioblastoma / Cell Culture Techniques / Neural Stem Cells Type of study: Guideline / Prognostic_studies Limits: Animals / Humans Language: En Journal: J Vis Exp Year: 2014 Document type: Article Country of publication: United States

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