Isolation and RFLP genotyping of Toxoplasma gondii from the mongoose (Herpestes auropunctatus) in Grenada, West Indies.
J Zoo Wildl Med
; 44(4): 1127-30, 2013 Dec.
Article
in En
| MEDLINE
| ID: mdl-24450085
ABSTRACT
Little is known of the genetic diversity and epidemiology of Toxoplasma gondii infection in wildlife in Caribbean Islands. The prevalence and genetic diversity of T. gondii in mongooses (Herpestes auropunctatus) was investigated. During 2011 and 2012, 91 mongooses were trapped in different parts of Grenada, bled, euthanized, and examined at necropsy. Antibodies to T. gondii were found in 27 mongooses tested by the modified agglutination test (cut-off titer 25). Muscles (heart, tongue, neck) of 25 of the seropositive mongooses were bioassayed for T. gondii infection in mice. Viable T. gondii was isolated by bioassay in mice from four mongooses with MAT titers of 150 in two, 1200 for one, and 1400 for one mongoose. The four T. gondii isolates were further propagated in cell culture. Strain typing of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed one isolate belongs to the Type III (ToxoDB #2) lineage, two to ToxoDB#7 lineage, and one to the ToxoDB #216 lineage. This is the first report of T. gondii isolation and genotyping in H. auropunctatus worldwide.
Search on Google
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Toxoplasma
/
Toxoplasmosis, Animal
/
Herpestidae
Type of study:
Risk_factors_studies
Limits:
Animals
Country/Region as subject:
Caribe
/
Caribe ingles
/
Grenada
Language:
En
Journal:
J Zoo Wildl Med
Journal subject:
MEDICINA VETERINARIA
Year:
2013
Document type:
Article
Affiliation country:
United States