Structural and kinetic studies on adenylosuccinate lyase from Mycobacterium smegmatis and Mycobacterium tuberculosis provide new insights on the catalytic residues of the enzyme.

Banerjee, Sanchari; Agrawal, Monika J; Mishra, Diptimayee; Sharan, Siddharth; Balaram, Hemalatha; Savithri, Handanhal S; Murthy, Mathur R N

Structural and kinetic studies on adenylosuccinate lyase from Mycobacterium smegmatis and Mycobacterium tuberculosis provide new insights on the catalytic residues of the enzyme.

Banerjee, Sanchari; Agrawal, Monika J; Mishra, Diptimayee; Sharan, Siddharth; Balaram, Hemalatha; Savithri, Handanhal S; Murthy, Mathur R N.

Affiliation

Banerjee S; Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.

FEBS J ; 281(6): 1642-58, 2014 Mar.

Article in En | MEDLINE | ID: mdl-24479855

Subject(s)

Adenylosuccinate Lyase/chemistry; Adenylosuccinate Lyase/metabolism; Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Mycobacterium smegmatis/enzymology; Mycobacterium tuberculosis/enzymology; Adenylosuccinate Lyase/genetics; Amino Acid Sequence; Apoenzymes/chemistry; Apoenzymes/genetics; Apoenzymes/metabolism; Bacterial Proteins/genetics; Catalytic Domain/genetics; Crystallography, X-Ray; Kinetics; Models, Molecular; Molecular Sequence Data; Mutagenesis, Site-Directed; Mycobacterium smegmatis/genetics; Mycobacterium tuberculosis/genetics; Phylogeny; Protein Conformation; Protein Structure, Quaternary; Sequence Homology, Amino Acid; Static Electricity; Structural Homology, Protein

Key words

X-ray crystallography; adenylosuccinate lyase; catalytic activity; purine nucleotide supply; slow growth rate

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Adenylosuccinate Lyase / Mycobacterium smegmatis / Mycobacterium tuberculosis Language: En Journal: FEBS J Journal subject: BIOQUIMICA Year: 2014 Document type: Article Affiliation country: India Country of publication: United kingdom

Fulltext

Add to My VHL

XML

PubMed Links

Search on Google