Discovery of human sORF-encoded polypeptides (SEPs) in cell lines and tissue.
J Proteome Res
; 13(3): 1757-65, 2014 Mar 07.
Article
in En
| MEDLINE
| ID: mdl-24490786
ABSTRACT
The existence of nonannotated protein-coding human short open reading frames (sORFs) has been revealed through the direct detection of their sORF-encoded polypeptide (SEP) products. The discovery of novel SEPs increases the size of the genome and the proteome and provides insights into the molecular biology of mammalian cells, such as the prevalent usage of non-AUG start codons. Through modifications of the existing SEP-discovery workflow, we discover an additional 195 SEPs in K562 cells and extend this methodology to identify novel human SEPs in additional cell lines and human tissue for a final tally of 237 new SEPs. These results continue to expand the human genome and proteome and demonstrate that SEPs are a ubiquitous class of nonannotated polypeptides that require further investigation.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptides
/
Breast Neoplasms
/
Genome, Human
/
Open Reading Frames
/
Proteome
Limits:
Female
/
Humans
Language:
En
Journal:
J Proteome Res
Journal subject:
BIOQUIMICA
Year:
2014
Document type:
Article
Affiliation country:
United States