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Optimized reverse micelle surfactant system for high-resolution NMR spectroscopy of encapsulated proteins and nucleic acids dissolved in low viscosity fluids.
Dodevski, Igor; Nucci, Nathaniel V; Valentine, Kathleen G; Sidhu, Gurnimrat K; O'Brien, Evan S; Pardi, Arthur; Wand, A Joshua.
Affiliation
  • Dodevski I; Johnson Research Foundation and Department of Biochemistry and Biophysics, University of Pennsylvania , Philadelphia, Pennsylvania 19104-6059.
J Am Chem Soc ; 136(9): 3465-74, 2014 Mar 05.
Article in En | MEDLINE | ID: mdl-24495164
ABSTRACT
An optimized reverse micelle surfactant system has been developed for solution nuclear magnetic resonance studies of encapsulated proteins and nucleic acids dissolved in low viscosity fluids. Comprising the nonionic 1-decanoyl-rac-glycerol and the zwitterionic lauryldimethylamine-N-oxide (10MAG/LDAO), this mixture is shown to efficiently encapsulate a diverse set of proteins and nucleic acids. Chemical shift analyses of these systems show that high structural fidelity is achieved upon encapsulation. The 10MAG/LDAO surfactant system reduces the molecular reorientation time for encapsulated macromolecules larger than ~20 kDa leading to improved overall NMR performance. The 10MAG/LDAO system can also be used for solution NMR studies of lipid-modified proteins. New and efficient strategies for optimization of encapsulation conditions are described. 10MAG/LDAO performs well in both the low viscosity pentane and ultralow viscosity liquid ethane and therefore will serve as a general surfactant system for initiating solution NMR studies of proteins and nucleic acids.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Surface-Active Agents / DNA / RNA / Dimethylamines / Membrane Proteins / Micelles Language: En Journal: J Am Chem Soc Year: 2014 Document type: Article Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Surface-Active Agents / DNA / RNA / Dimethylamines / Membrane Proteins / Micelles Language: En Journal: J Am Chem Soc Year: 2014 Document type: Article Publication country: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA