Deficient DNA binding of an apurinic/apyrimidinic DNA endonuclease activity from xeroderma pigmentosum cells.
Cell Biol Int Rep
; 12(3): 231-7, 1988 Mar.
Article
in En
| MEDLINE
| ID: mdl-2454750
ABSTRACT
A chromatin-associated apurinic/apyrimidinic (AP) DNA endonuclease activity, pI 9.8, from both normal human and xeroderma pigmentosum, complementation group A (XPA), lymphoblastoid cells was examined for its ability to bind AP DNA using a filter binding assay. The endonuclease activity from normal cells produced significantly greater binding to AP DNA than to untreated DNA, but this increase in binding was not observed when the XPA endonuclease was incubated with AP DNA versus untreated DNA. These results indicate that the XPA AP endonuclease activity is deficient in its ability to bind to AP DNA.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Xeroderma Pigmentosum
/
DNA
/
Endodeoxyribonucleases
Limits:
Humans
Language:
En
Journal:
Cell Biol Int Rep
Year:
1988
Document type:
Article