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Mycobacterium tuberculosis-specific polyfunctional cytotoxic CD8+ T cells express CD69.
Li, Li; Yang, Binyan; Zhang, Xianlan; Lao, Suihua.
Affiliation
  • Li L; Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China.
  • Yang B; Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China.
  • Zhang X; Chest Hospital of Guangzhou, Guangzhou 510095, PR China.
  • Lao S; Chest Hospital of Guangzhou, Guangzhou 510095, PR China.
  • Changyou Wu; Institute of Immunology, Zhongshan School of Medicine, Key Laboratory of Tropical Disease Control Research of Ministry of Education, Sun Yat-sen University, Guangzhou 510080, PR China. Electronic address: changyou_wu@yahoo.com.
Tuberculosis (Edinb) ; 94(3): 219-25, 2014 May.
Article in En | MEDLINE | ID: mdl-24566284
Increasing evidences in animals and humans suggest that CD8(+) T cells contribute significantly to immune defenses against Mycobacterium tuberculosis (Mtb). In the present study, we found that without any stimulation, CD8(+) T cells in pleural fluid cells (PFCs) expressed significantly higher levels of CD69 than PBMCs from patients with tuberculous pleurisy (TBP). CD8(+)CD69(+) T cells expressed significantly higher levels of CD45RO and HLA-DR and lower levels of CD45RA than CD8(+)CD69(-) T cells, demonstrating that CD8(+)CD69(+) T cells were activated memory cells. Furthermore, we found higher expression of CCR6 and lower expression of CCR7 and CD62L on CD8(+)CD69(+) T cells compared with CD8(+)CD69(-) T cells, suggesting that the expression of CCR6 and reduced expression of CCR7 and CD62L might facilitate the migration of circulating CD8(+)CD69(+) T cells into tuberculous pleural space. Importantly, following stimulation with culture filtrate protein of 10 kDa (CFP10) peptides, CD8(+)CD69(+) T cells but not CD8(+)CD69(-) T cells expressed CD107a/b, IFN-γ and TNF-α, demonstrating that CD8(+)CD69(+) T cells were MTB-specific cells. In addition, the majority of CD8(+)CD69(+) T cells were dominated by polyfunctional T cells. In summary, we demonstrated that CD69 as a useful marker for MTB-specific CD8(+) T cells in PFCs from patients with TBP enabled a direct ex vivo estimation of the quantity, as well as the quality, of MTB-specific CD8(+) responses.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tuberculosis, Pleural / Antigens, Differentiation, T-Lymphocyte / Antigens, CD / CD8-Positive T-Lymphocytes / Lectins, C-Type Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: En Journal: Tuberculosis (Edinb) Year: 2014 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Tuberculosis, Pleural / Antigens, Differentiation, T-Lymphocyte / Antigens, CD / CD8-Positive T-Lymphocytes / Lectins, C-Type Limits: Adult / Aged / Female / Humans / Male / Middle aged Language: En Journal: Tuberculosis (Edinb) Year: 2014 Document type: Article Country of publication: United kingdom