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Microfluidic-based manufacture of siRNA-lipid nanoparticles for therapeutic applications.
Walsh, Colin; Ou, Kevin; Belliveau, Nathan M; Leaver, Tim J; Wild, Andre W; Huft, Jens; Lin, Paulo J; Chen, Sam; Leung, Alex K; Lee, Justin B; Hansen, Carl L; Taylor, Robert J; Ramsay, Euan C; Cullis, Pieter R.
Affiliation
  • Walsh C; University of British Columbia, Vancouver, BC, Canada.
Methods Mol Biol ; 1141: 109-20, 2014.
Article in En | MEDLINE | ID: mdl-24567134
ABSTRACT
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphatidylcholines / Cholesterol / Drug Delivery Systems / RNA, Small Interfering / Microfluidics / Nanoparticles Type of study: Guideline Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2014 Document type: Article Affiliation country: Canada
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphatidylcholines / Cholesterol / Drug Delivery Systems / RNA, Small Interfering / Microfluidics / Nanoparticles Type of study: Guideline Limits: Animals / Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2014 Document type: Article Affiliation country: Canada