Microfluidic-based manufacture of siRNA-lipid nanoparticles for therapeutic applications.
Methods Mol Biol
; 1141: 109-20, 2014.
Article
in En
| MEDLINE
| ID: mdl-24567134
ABSTRACT
A simple, efficient, and scalable manufacturing technique is required for developing siRNA-lipid nanoparticles (siRNA-LNP) for therapeutic applications. In this chapter we describe a novel microfluidic-based manufacturing process for the rapid manufacture of siRNA-LNP, together with protocols for characterizing the size, polydispersity, RNA encapsulation efficiency, RNA concentration, and total lipid concentration of the resultant nanoparticles.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphatidylcholines
/
Cholesterol
/
Drug Delivery Systems
/
RNA, Small Interfering
/
Microfluidics
/
Nanoparticles
Type of study:
Guideline
Limits:
Animals
/
Humans
Language:
En
Journal:
Methods Mol Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2014
Document type:
Article
Affiliation country:
Canada