Quantification of oligonucleotides by LC-MS/MS: the challenges of quantifying a phosphorothioate oligonucleotide and multiple metabolites.

Ewles, Matthew; Goodwin, Lee; Schneider, Anneliese; Rothhammer-Hampl, Tanja

Ewles, Matthew; Goodwin, Lee; Schneider, Anneliese; Rothhammer-Hampl, Tanja.

Affiliation

Ewles M; Covance Laboratories Ltd, Otley Road, Harrogate, HG3 1PY, UK.

Bioanalysis ; 6(4): 447-64, 2014 Feb.

Article in En | MEDLINE | ID: mdl-24568349

Subject(s)

Chromatography, High Pressure Liquid; Phosphorothioate Oligonucleotides/blood; Tandem Mass Spectrometry; Calibration; Chloroform/chemistry; Chromatography, High Pressure Liquid/standards; Humans; Metabolome; Oligodeoxyribonucleotides/blood; Oligodeoxyribonucleotides/isolation & purification; Oligodeoxyribonucleotides/metabolism; Phenol/chemistry; Phosphorothioate Oligonucleotides/isolation & purification; Phosphorothioate Oligonucleotides/standards; Solid Phase Extraction; Tandem Mass Spectrometry/standards; Thionucleotides/blood; Thionucleotides/isolation & purification; Thionucleotides/metabolism

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromatography, High Pressure Liquid / Tandem Mass Spectrometry / Phosphorothioate Oligonucleotides Limits: Humans Language: En Journal: Bioanalysis Year: 2014 Document type: Article Affiliation country: United kingdom Country of publication: United kingdom

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