High detection rates of enteropathogens in asymptomatic children attending day care.
PLoS One
; 9(2): e89496, 2014.
Article
in En
| MEDLINE
| ID: mdl-24586825
ABSTRACT
BACKGROUND:
Gastroenteritis morbidity is high among children under the age of four, especially amongst those who attend day care.OBJECTIVE:
To determine the prevalence of a range of enteropathogens in the intestinal flora of children attending day care and to relate their occurrence with characteristics of the sampled child and the sampling season.METHODS:
We performed three years of enteropathogen surveillance in a network of 29 child day care centers in the Netherlands. The centers were instructed to take one fecal sample from ten randomly chosen children each month, regardless of gastrointestinal symptoms at time of sampling. All samples were analyzed for the molecular detection of 16 enteropathogenic bacteria, parasites and viruses by real-time multiplex PCR.RESULTS:
Enteropathogens were detected in 78.0% of the 5197 fecal samples. Of the total, 95.4% of samples were obtained from children who had no gastroenteritis symptoms at time of sampling. Bacterial enteropathogens were detected most often (most prevalent EPEC, 19.9%), followed by parasitic enteropathogens (most prevalent D. fragilis, 22.1%) and viral enteropathogens (most prevalent norovirus, 9.5%). 4.6% of samples related to children that experienced symptoms of gastroenteritis at time of sampling. Only rotavirus and norovirus were significantly associated with gastroenteritis among day care attendees.CONCLUSIONS:
Our study indicates that asymptomatic infections with enteropathogens in day care attendees are not a rare event and that gastroenteritis caused by infections with these enteropathogens is only one expression of their presence.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Parasitic Diseases
/
Rotavirus Infections
/
Carrier State
/
Child Day Care Centers
/
Enterobacteriaceae
/
Enterobacteriaceae Infections
/
Feces
Type of study:
Diagnostic_studies
/
Prevalence_studies
/
Risk_factors_studies
Limits:
Animals
/
Child, preschool
/
Female
/
Humans
/
Male
Country/Region as subject:
Europa
Language:
En
Journal:
PLoS One
Journal subject:
CIENCIA
/
MEDICINA
Year:
2014
Document type:
Article
Affiliation country:
Netherlands