Measurement of intracellular Ca2+ release in permeabilized cells using 45Ca2+.
Cold Spring Harb Protoc
; 2014(3): 289-94, 2014 Mar 01.
Article
in En
| MEDLINE
| ID: mdl-24591686
ABSTRACT
This protocol describes a technique to measure Ca(2+) release from the nonmitochondrial intracellular Ca(2+) stores in monolayers of saponin-permeabilized cells cultured in 12-well 4-cm(2) clusters. The (45)Ca(2+)-flux technique described here can only be applied to cell types that still adhere to the plastic after exposing them to saponin. We describe the permeabilization procedure, the loading of the nonmitochondrial Ca(2+) stores with (45)Ca(2+), and the subsequent (45)Ca(2+) efflux.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Calcium Radioisotopes
/
Cell Membrane Permeability
/
Calcium
Language:
En
Journal:
Cold Spring Harb Protoc
Year:
2014
Document type:
Article
Affiliation country:
Belgium